化合物溶解度列在产品网页、产品信息表或产品安全和数据表中。查看产品信息表以了解制备储备液的具体说明。储备溶液通常按最终所需工作浓度的 10X-1000X 制备，通常在 1-100 mg/mL 的范围内。对于染料和指示剂，我们通常在推荐的溶剂中制备浓度为 5-10 mM 的浓缩储备液。然后可以在用于应用的缓冲液或介质中将染料稀释至最终所需浓度。

溶解度定义

要溶解冻干化合物，只需将适当体积的推荐溶剂添加到小瓶中，制成所需浓度的储备溶液，然后旋转或轻轻涡旋混合。确保溶剂与小瓶的内壁接触以完全回收产品。

难以溶解的化合物可加热至 50°C 或更高（注意：我们不建议加热腔肠素溶液），并涡旋或超声处理直至完全溶解。一些化合物只是在动力学上缓慢溶解。当化合物是高纯度和结晶形式时尤其如此。另一种更温和的溶解这些化合物的方法是在黑暗中简单地摇晃化合物/溶剂混合物过夜。

When preparing stock solutions in organic solvents such as DMF, DMSO, or alcohol for use in living cells or organisms, make a concentrated stock so that the final concentration of solvent in the working solution will not be toxic. A general guideline for immortalized cell lines is to keep the final solvent concentration below 1%; certain cell lines, primary cells or experimental organisms may be more sensitive to solvent toxicity. Similarly, for enzymatic reactions, the final concentration of solvent should be kept below 1% to avoid inhibiting enzyme activity.

Compounds dissolved in aqueous solution can be sterilized by filtration if necessary. Generally, concentrated stock solutions of compounds in organic solvents do not require sterilization, but culture medium can be sterilized by filtration after addition of the compound.