AccuClear®、AccuBlue® 和 AccuGreen™ DNA 定量试剂盒使用高分子量 dsDNA 标准品，在定量高分子量 DNA 时最为准确。虽然所有 DNA 定量分析都在某种程度上对 DNA 长度敏感，但某些分析对短 dsDNA 片段的分析比其他分析更准确。

对于 25 到 500 bp 之间的片段定量：

• AccuBlue® 高灵敏度检测是最准确的；片段的荧光约为基因组 DNA 荧光的 80%，并且在长度和浓度上一致。

• AccuBlue® Broad Range assay is also consistent across length and concentration, with ~ 60% of the fluorescence of genomic DNA.

• AccuGreen™ High Sensitivity assay gives a consistent signal of ~ 80% of the fluorescence of genomic DNA with fragments of 100 bp and higher, but only in the range of 10-100 ng of DNA. Below that, small DNA fragments were inconsistently quantified.

• AccuClear® assay is more variable based on DNA fragment length and concentration. Therefore we do not recommend using this assay with short dsDNA fragments.

To quantify short dsDNA fragments we recommend:

• Use the AccuBlue® High Sensitivity DNA Quantitation assay

• Use sample concentrations well above the stated lower detection limit of the assay (for example, if the stated linear detection range is 0.2-100 ng, for a small fragment you should aim for an amount greater than 2 ng).

• （为获得最佳结果）提供您自己的 dsDNA 标准，其长度与您的样本相似。